Probing and pressing surfaces of hepatitis C virus-like particles

S Collett; J Torresi; L Earnest-Silveira; D Christiansen; A Elbourne; PA Ramsland

Journal article

Probing and pressing surfaces of hepatitis C virus-like particles

S Collett, J Torresi, L Earnest-Silveira, D Christiansen, A Elbourne, PA Ramsland

Journal of Colloid and Interface Science | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2019

DOI: 10.1016/j.jcis.2019.03.022

Abstract

Hepatitis C virus-like particles (VLPs) are being developed as a quadrivalent vaccine candidate, eliciting both humoral and cellular immune responses in animal trials. Biophysical, biomechanical and biochemical properties are important for virus and VLP interactions with host cells and recognition by the immune system. Atomic force microscopy (AFM) is a powerful tool for visualizing surface topographies of cells, bionanoparticles and biomolecules, and for determining biophysical and biomechanical attributes such as size and elasticity. In this work, AFM was used to define morphological and nanomechanical properties of VLPs representing four common genotypes of hepatitis C virus. Significant ..

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University of Melbourne Researchers

Simon Collett Author

Joseph Torresi Author

Linda Earnest Author

Paul Ramsland Author

Related Projects (2)

DEVELOPING A QUADRIVALENT HCV VACCINE

A vaccine for hepatitis C virus (HCV) is not yet available. Immune responses that are able to protect against infection are possible, making..

HIGH RESOLUTION ATOMIC FORCE MICROSCOPY FACILITY FOR BIONANOTECHNOLOGY

This project aims to establish a collaborative high resolution atomic force microscopy facility. Nanoscale surface structure and the complex..

Grants

Awarded by Australian Research Council

Funding Acknowledgements

The authors thank Dr Andrew J. Guy and Dr Madeleine F. Dupont (RMIT University) for advice and support for computational implementation and statistical analysis. SC is supported by a research training program stipend scholarship from the Australian Government, Department of Education and Training. AFM work was conducted in the MicroNano Research Facility (MNRF) at RMIT University and the Cypher ES AFM instrument was funded in part by grant LE170100096 from the Australian Research Council (ARC). This work was supported by the National Health and Medical Research Council (NHMRC) of Australia, grant number 1126379. JT is supported by an NHMRC Practitioner Fellowship, number 106043.